动物造模,皮肤型狼疮,药效评价 zi-bio 2024-10-07 743

　　Objective: To observe and identify the skin symptoms of aged systemic lupus erythematosus (TC) mice and explore whether it can be used as a mouse model for studying cutaneous lupus

　　Method: Observe the age at which TC mice experience skin symptoms, compare the skin symptoms of mice of the same age with C57BL/6 mice, detect serum anti dsDNA antibodies and anti ANA antibodies using ELISA, and detect pathological symptoms of mouse skin using HE staining

　　Result: 2/3 of TC mice showed symptoms such as hair loss and skin ulceration, with the earliest onset of lesions occurring at 40 weeks of age. The serum anti dsDNA antibody and anti ANA antibody titers of lupus mice with skin lesions were significantly higher than those of C57BL/6 mice (P<0.05); The HE staining results showed that the stratum corneum of lupus mice was missing, the continuity of the epithelial layer was interrupted, and a large number of lymphocytes infiltrated the dermis

　　Conclusion: Lupus mice exhibit skin lesions after 40 weeks, indicating that this mouse can serve as a disease model for studying chronic cutaneous lupus