动物造模,NAFLD模型 zi-bio 2024-03-06 641

　　In the post genomic era, the long clawed gerbil also needs to conduct in-depth research with new technologies in order to achieve sustainable development. The animal models currently used have various shortcomings to varying degrees. Our main goal is to seek a method that is extremely similar to human disease progression, with simple and easy implementation, high modeling rate, and the ability to identify genetic markers for inbred line breeding based on the evaluation of the genetic structure of closed populations.

　　This study prepared a simple feed and established a rapid induced NAFLD model for the first time. Long term observation was conducted on a closed group, and an elderly spontaneous model was discovered for the first time. Clinical commonly used serum electrophoresis detection technology was used to classify and evaluate the model. At the same time, PCR DGGE technology was established to evaluate the gut microbiota of the model; Secondly, transcriptome sequencing technology was used to perform in-depth sequencing on the induced and spontaneous models of long clawed gerbils, identifying some highly expressed genes and significantly different pathways compared to the rapid and spontaneous models of long clawed gerbils; Thirdly, starting from classical theories and closely related genes, the candidate gene method and immunohistochemistry method are used to predict function to evaluate the correlation between genes and hyperlipidemia in gerbils; Finally, a large sample was taken from the ZCLA closed population, covering two microbial grade production groups. The genetic diversity of the selected gene SNPs was evaluated to determine their potential as NAFLD genetic markers for inbred line breeding.