动物造模,慢性肾病 zi-bio 2024-02-27 754

　　Objective: To establish and evaluate a rat model of chronic obstructive pulmonary disease combined with chronic kidney disease.

　　Method: Forty male and female SPF grade SD rats were randomly divided into a control group (Control group), COPD group, CKD group, and COPD combined with CKD model group (COPD+CKD group), with 10 rats in each group. A COPD rat model was prepared using cigarette smoke exposure combined with bacterial infusion, and a CKD rat model was established using adenine induction. The two methods collaborated to prepare a COPD combined with CKD rat model. After 8 weeks, samples were taken to test lung and kidney function, and tissue morphology and serum inflammatory cytokine expression levels were observed.

　　After successful modeling, the lung function indicators FVC, FEV01, and FEV01/FVC in the COPD+CKD group of rats were significantly reduced (P<001). The pathological manifestations of lung tissue were alveolar wall rupture, fused emphysema changes, and inflammatory cell infiltration; Blood Cr, BUN, and 24-hour urine protein significantly increased (P<001), and renal tissue pathology showed glomerular mesangial hyperplasia, thickening of the basement membrane, renal tubular dilation, and interstitial fibrosis. Ultrastructure showed partial closure of glomerular capillary loops, fusion of foot processes, fusion and disintegration of renal tubular mitochondria, and increased lysosomes; Serum IL-6, IL-13, IL-1 β And TGF ⁃ β 1 level was significantly higher than that of single model group (P.

　　Conclusion: The method of combining cigarette smoke exposure with bacterial infection and 25% adenine induction can successfully construct a rat model of COPD combined with CKD, and inflammatory response may play a key role in the process of COPD combined with CKD.