动物造模,肝损伤 zi-bio 2024-02-18 704

　　1. Animal modeling materials: Healthy adult guinea pigs, weighing 250-400g, regardless of gender; Medication: Freund's complete adjuvant; Instrument: homogenizer.

　　2. The modeling method involves heparinizing guinea pigs under sterile conditions and perfusing them with physiological saline through the portal vein to wash away blood in the liver until it displays its inherent color. Take out the liver and cut it into pieces, then wash it repeatedly with physiological saline to further wash away the blood. Take 10g of wet liver tissue and add 100ml of physiological saline to prepare a 10% liver homogenate physiological saline suspension in a homogenizer. Add an equal amount of Freund's complete adjuvant to the liver suspension to make an emulsion. Inject 2ml of emulsifier into the abdominal cavity of each animal (or inject 2ml intramuscularly, or inject 0.1ml of each quadruped pad). Regular feeding.

　　3. Modeling principle: Animal liver injury model caused by homologous immunity.

　　4. After injection for about one month, granulomatous lesions of liver tissue, edema like degeneration, eosinophilic degeneration and necrosis of liver cells, proliferation of bile duct epithelium, and lymphocyte infiltration can be observed. The above lesions are similar to chronic active hepatitis.