动物造模,肝损伤 zi-bio 2024-02-18 665

　　1. Animal modeling materials: hybrid rats or mice, newly weaned male purebred mice; Medication: Freund's complete adjuvant; Equipment: High speed freezer centrifuge, homogenizer.

　　2. Modeling method: The hybrid mice were decapitated and euthanized. Blood was washed from the liver via the portal vein with physiological saline, and the liver was cut into small pieces and soaked in physiological saline until colorless. Take 10g of wet liver tissue and add 100ml of physiological saline to a homogenizer to make a 10% liver homogenate. Then, centrifuge at low temperature for 30 minutes (0-4 ℃) using a high-speed freezing centrifuge at 10000 × g, and add an equal amount of Freund's complete adjuvant to the supernatant to make an emulsion. Inject 0.1ml of emulsion subcutaneously into the back of newly weaned male purebred mice for immunization, twice a week for a total of 5 weeks, and then change to once a week for a total of 4 weeks, for a total of 14 times before and after.

　　3. Principle of modeling: Animal liver injury model caused by heterologous immunity.

　　4. After modeling, liver pathological examination was performed on dead mice at 2, 5, and 14 weeks after immunization. Neutrophil and monocyte infiltration was found in the portal vein area of the liver, with turbid liver cells and scattered punctate necrosis, extensive proliferation of stellate cells, and obvious splenomegaly.