动物造模,肝硬化 zi-bio 2024-02-18 766

　　1. Animal modeling materials: Wistar or SD rats, weighing around 180-200g; Medication: 40% to 50% carbon tetrachloride oil solution, 20% to 30% alcohol, pure corn flour (mixed with 0.5% cholesterol).

　　2. Method of modeling: The modeling animals were subcutaneously injected with 40% to 50% carbon tetrachloride oil solution (0.3ml/100g) twice a week. Starting from the second week, 1ml of 20% to 30% alcohol was orally administered (or used as the only beverage) every other day, and pure corn flour (mixed with 0.5% cholesterol) was fed for a total of 10 weeks.

　　3. Modeling principle: Carbon tetrachloride leads to high cholesterol diet induced liver cirrhosis in rats.

　　4. Changes after modeling: At the second week of modeling, the liver showed degeneration and necrosis of small lobular liver cells, and no obvious fibrous proliferation was observed under the light microscope; At the fourth week, in addition to hepatocyte degeneration and necrosis, thin fibrous septa begin to form in the liver; At week 6, the liver septum of rats further thickened and many pseudolobules formed; At week 8, the normal structure of liver tissue in rats was disrupted, forming thick fibrous septa and dividing into pseudolobules.