动物造模,肝纤维化 zi-bio 2024-02-18 706

　　1. Animal modeling materials: macaques, male, aged 7-10 years old, weighing 8-10kg; Drug: Analytical pure CCl4.

　　2. Modeling method: Animals were raised and observed for 30 days without any abnormalities, and biochemical indicators of heart, liver, and kidney were tested without any abnormalities. Randomly divide the animals into a model group and a control group.

　　Analyze pure CCl4, prepare 400ml/L, 0.8ml/kg with olive oil, intravenous injection once a week, supplemented by high-fat feed, and continuously treat for 10 weeks.

　　3. The principle of modeling is to inject CCl4 to cause liver cell degeneration and necrosis, while supplementing with high-fat feed to increase liver load and induce liver tissue fibrosis.

　　4. After modeling, the normal liver tissue structure is clear, liver cells are arranged neatly, liver lobules are intact, and liver cells are arranged radially around the central vein. The liver tissue of model animals shows punctate or focal hepatocyte necrosis and immune cell infiltration, fibrous proliferation between liver lobules, and some fibrous cells extending into the liver cell space.

　　Both serum AST and ALT were significantly elevated; The content of serum total protein (TP) and albumin decreased, while the content of globulin (GLO) increased; The content of total cholesterol esters, triglycerides, and low-density lipoprotein in serum increases, while the content of high-density lipoprotein decreases. The content of total bilirubin, direct bilirubin, and indirect bilirubin increases. The content of serum hyaluronic acid (HA), type III procollagen (PC III), and type IV collagen is significantly higher than that of the healthy control group.