动物造模,肾血管性高血压动物模型 z-bio 2023-11-27 1233

　　1、 Overview of Renal Vascular Hypertension

　　Renal vascular hypertension is the most common secondary hypertension caused by narrowing or obstruction of one or both renal artery trunks or branches, resulting in reduced renal vascular flow and renal ischemia. Renal vascular hypertension accounts for 2% to 7% of the hypertensive population in China.

　　Renal vascular hypertension is caused by the activation of the renin angiotensin aldosterone system due to ischemia, and the activation of the renin angiotensin aldosterone system has been recognized as the pathogenesis of hypertension. Therefore, animal renal artery stenosis can very similarly replicate a hypertensive model. In 1934, Goldblatt confirmed that narrow renal arteries in dogs can cause persistent hypertension, opening up a pathway for experimental research on hypertension. The animal model of renovascular hypertension in rats is easy to replicate and has sufficient animal sources, making it a commonly used animal model for studying the mechanism of hypertension and drug experiments.

　　2、 Preparation of renal artery stenosis animal model

　　Currently. The rat model of renal vascular hypertension can be divided into two types according to different modeling methods: ① Two kidneys and one clip renal vascular hypertension rat model; ② Single kidney single clip renal vascular hypertension rat model; ③ Double kidney and double clip. Renal vascular hypertension rat model.

　　（1） A rat model of renal vascular hypertension with two (dual) kidneys and one clip

　　1. Method: Healthy male SD rats, weighing 180-220g, were anesthetized with pentobarbital sodium. The back was fixed on a wooden board to expose the abdomen, and a midline incision was made into the abdominal cavity to separate and expose one side of the renal artery. A silver clip or ring with an inner diameter of 0.2mm was used to cover the renal artery and cause renal artery stenosis. If the postoperative systolic blood pressure is higher than 140mmHg, it is determined that the establishment of a two kidney one clip renal vascular hypertension animal model is successful.

　　2. Evaluate that this model has the advantages of simple modeling, high success rate, and strong homogeneity. It is comparable to the pathological process of human hypertension and is the most commonly used classic animal model for hypertension internationally. It is currently one of the most commonly used models for screening antihypertensive drugs.

　　（2） The dual kidney double clip renal vascular hypertension rat model is also known as a stroke prone renal vascular hypertension rat model due to its spontaneous stroke incidence rate of up to 56.4%.

　　1. Method: Healthy male SD rats, weighing 120-240g, were anesthetized by intraperitoneal injection of 3% pentobarbital sodium for 10-15 minutes. Under aseptic operation, bilateral renal arteries were bluntly separated through a longitudinal incision in the middle of the abdomen, and the starting points of bilateral renal arteries were clamped with self-made circular silver clips with an inner diameter of 0.2mm. The entire surgery does not damage the kidneys, liver, chyle pool, or. Renal vein. Postoperative intraperitoneal injection of a small amount of penicillin to prevent infection. The blood pressure of male SD rats before the experiment was (110 ± 9) mmHg, which increased to (124.5 ± 11.2) mmHg one week after surgery, significantly higher than the preoperative blood pressure level. At three weeks after surgery, the blood pressure exceeded 150.0mmHg, and some rats may have blood pressure higher than 250mmHg.

　　2. Evaluation: This model is the first hypertensive rat model in China and is currently being promoted and used domestically. It is a very promising and applicable model