动物造模,高原红细胞增多症 zi-bio 2023-11-23 883

　　High altitude polycythemia (referred to as "hypererythrosis") is a chronic high altitude disease caused by excessive compensatory proliferation of red blood cells (i.e., excessive proliferation of red blood cells) caused by high altitude hypoxia. Compared with healthy individuals at the same altitude, patients with hyperemia have significantly increased red blood cell, hemoglobin, and red blood cell volume, decreased arterial oxygen saturation, and accompanied by clinical symptoms and signs of polyemia. This disease is mostly found in migrants at high altitude, but rarely in natives at high altitude. The incidence rate of men is significantly higher than that of women, and children are rare. A rat model of high altitude polycythemia disease can be replicated using a combination of factors such as hypoxia, high cold, and oral cobalt chloride.

　　Model making method: SD rats, weighing 180-220g, male. Place the model group rats in a "plateau environment simulation chamber". The cabin is equipped with three boxes of ice cubes, and is simulated in a high-altitude environment of high cold and low oxygen using decompression and hypoxia methods. The temperature inside the cabin drops (9.0-11.0) ℃ compared to indoors. Close the cabin door tightly and use a vacuum pump for pressure reduction. During the pressure reduction process, it is necessary to adjust the airflow at the inlet to maintain fresh air with relative wind speed inside the cabin. Perform stepwise intermittent decompression according to the altitude indicated by the "plateau empty box barometer" inside the cabin. Decompression takes about 6 hours per day, and animals are immediately given a 15mg/kg body weight solution of cobalt chloride by gavage after leaving the cabin. Sunday is a rest day for 40 consecutive days. The control group rats ate freely at room temperature.

　　Observation indicators and analysis:

　　1. Measurement of hemoglobin content, hematocrit, and number of red blood cells;

　　2. Whole blood viscosity measurement;

　　3. Observation of the impact of different modeling cycles on modeling and the possible duration of disease model formation under 2260 meter environmental conditions;

　　4. Pathological examination of the lungs and kidneys.