动物造模,急性心理应激模型 zi-bio 2023-11-23 837

　　Model production method:

　　SD rat, 8 weeks old, male. The laboratory temperature is between 18 and 25 ℃, with regular feeding and free diet.

　　1. Exercise mode and conditions: weightless swimming, glass tank swimming pool, water depth about twice the length of rats, and water temperature of 30-32 ℃.

　　2. Preparation of a psychological stress model: Using a rat bystander electric shock model, rats in the electric shock group (with a current of 1-2mA, a cycle of 100 seconds, and an interval of 90 seconds) were shocked and startled by the electric shock; The rats in the psychological stress model group were not subjected to electric shock, but only observed the process of electric shock in the shock group. Psychological stress was generated through visual, auditory, and other means, and was carried out in batches at the end of the experiment, lasting for 30 minutes.

　　3. Group domain experimental control includes: a, control group: no exercise or stress; b. Acute psychological stress group: not exercising, but receiving psychological stress; c. 30 minute swimming group: In the first week, adaptive swimming was performed daily, gradually increasing from 10 minutes to 30 minutes. Starting from the second week, swimming was performed once a day for 30 minutes each time, with a scheduled rest once a week for a total of 11 weeks; d. 60 minute swimming group: In the first week, adaptive swimming exercise was performed daily, gradually increasing from 10 minutes to 60 minutes. Starting from the second week, swimming exercise was performed once a day for 60 minutes each time, with regular rest once a week for a total of 11 weeks; e. (30 minute swimming+acute psychological stress) group: The exercise group was subjected to 30 minute exercise and psychological stress was applied at the end of the experiment; f. (60min swimming+acute psychological stress) group: Exercise mode: 60min exercise group, stress was applied at the end of the experiment.

　　Observation indicators and analysis:

　　1. Determination of serum IL-6 content;

　　2. Determination of serum TNF-a content.