动物造模,结膜炎模型 z-bio 2023-11-22 1143

　　Inoculation of Staphylococcus aureus into the conjunctiva scratch of the lower eyelid of rabbits causes infectious inflammation of the conjunctiva.

　　1. Model making method: New Zealand white rabbits, weighing 2.2-2.5kg, both male and female. Inoculate Staphylococcus aureus on blood agar medium 1 day before the experiment, incubate at 37 ℃ for 18 hours, and dilute with physiological saline to form Staphylococcus aureus 3 × 109 pieces/ml, spare. Take healthy New Zealand white rabbits without eye diseases and use sterile No. 9 needle to make a cross mark on the conjunctiva of the lower eyelid of the rabbit, preferably to cut it. Gently pull the eyelids into a small cup shape, and use a 0.25ml syringe to drip 0.1ml of bacterial solution into the conjunctival scar. After 24 hours of infection, each rabbit eye was randomly divided into groups according to the degree of inflammation, and treatment was given continuously for 4 days. Rabbit eye inflammation scores were given every 24 hours and statistically analyzed. The scoring criteria for conjunctivitis lesions are: no abnormalities in blood vessels, 0 point; Vascular congestion appears bright red with slight edema and a small amount of secretion, 1 point; Vascular congestion is purplish red in color, and blood vessels are not suitable for differentiation. There is obvious edema, and some of the eyelids are ectropion. The secretion makes the eyelids moist or sticky. 2 points; Diffuse congestion appears purple, with edema until the eyelids are almost closed, and secretions make the entire eyelids moist or sticky, with a score of 3.

　　2. Observation indicators and analysis

　　(1) Symptom observation: After inoculation with bacterial infection on the eyelids of animals, inflammatory reactions, conjunctival congestion, edema, and increased secretion were observed.

　　(2) Bacterial culture examination: conducted on the first day after inoculation of bacteria on the eyelids, the first day and fourth day after administration. Gently wipe the upper and lower eyelids with sterile saline wet cotton balls, then pour 5ml of physiological saline and mix evenly (test solution). Take 0.5ml of test solution and add it to the prepared ordinary agar culture medium plate. Spread evenly and then add it to a 37 ℃ incubator for 18 hours to determine whether the bacterial culture is negative or positive. The bacterial negative conversion rates of the model group after 1 day, 2 days, 5 days, and 8 days of eyelid inoculation were approximately 0, 33.3%, 75.0%, and 66.7%, respectively.

　　(3) Conjunctival pathology examination: On the 4th or 5th day after inoculation with bacteria, animals were euthanized and conjunctival tissue was taken for pathological observation. The model group animals had conjunctival dilation, congestion, and edema.