动物造模,失神经腓肠肌动物模型 z-bio 2023-11-21 637

　　1. Animal modeling materials: Healthy adult male SD rats, 150-200g; Medication: 1% pentobarbital.

　　2. Model making method: Model animals were anesthetized by intraperitoneal injection of 1% pentobarbital and underwent surgery to expose the sciatic and tibial nerves. The tibial nerves were ligated before the muscle entry point, and the tibial nerves were cut off at the proximal end of the ligation site. The proximal end was folded back and sutured to embed the gluteal muscles. An experimental model of peripheral nerve injury and denervation of the gastrocnemius muscle caused by a distal tibial nerve length of approximately 2.0cm.

　　3. The principle of modeling is to cut off the right tibial nerve in rats to form an experimental model of denervated gastrocnemius muscle.

　　4. Changes after modeling: Three months after tibial nerve transection, in the model group, the nerve fibers were thin and few in number, and there was a large proliferation of connective tissue between the bundles. The gastrocnemius muscle was thin, inelastic, and had a dull color. The volume of the gastrocnemius muscle decreased, the wet weight of the muscle decreased, and the content of actin decreased, showing denervation.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections. Surgical trauma should be minimized and sterile operations should be strictly carried out.