动物造模,急性坏死性胰腺炎 z-bio 2023-11-10 949

　　1. Model making material animals: SD rats, weighing 250-350g, regardless of gender; Medications: Sodium taurocholate, bioprotein gel, ketamine.

　　2. Modeling method: Experimental mice fasted 12 hours before surgery and drank freely. 1% ketamine is anesthetized by intraperitoneal injection at a dose of 1ml/100g body weight. Under sterile conditions, make a midline incision of about 5cm in the upper abdomen, search for the descending part of the duodenum, and identify the direction of the biliary and pancreatic ducts. Under the surgical microscope, the biliary and pancreatic ducts near the hilar segment are first clipped with small artery clamps, and the junction of the biliary and pancreatic ducts into the duodenum is also clipped with small artery clamps. Puncture the biliopancreatic duct from the outside of the intestine at the junction of the biliopancreatic duct and the duodenum with a No. 4 needle. After successful puncture, connect a micro pump and stably and uniformly inject 5% sodium taurocholate (0.1ml/100g) into the retrograde direction. After 2 minutes of injection, quickly seal the puncture opening with biological protein glue and keep it for about 10 minutes. After the egg white glue solidifies and there is visible bleeding and swelling in the pancreatic tissue, remove the two arterial clamps, confirm that the biliopancreatic duct is unobstructed and there is no bile leakage, and then close the abdomen layer by layer. After surgery, compound sodium lactate solution was injected subcutaneously, 4ml/100g, once/8 hours. The control group only underwent pancreatic flap and closed the abdomen.

　　3. Modeling principle: Retrograde injection of sodium taurocholate into the pancreatic duct leads to necrotizing pancreatitis.

　　4. Changes after modeling 72 hours after onset, the mortality rate in the model group was 70%, while in the sham surgery group it was 0. After 24 hours, compared with the sham surgery group (0.55 ± 0.09) ml, the weight of ascites in the model group (7.10 ± 1.08) ml significantly increased; Compared with the sham surgery group (229 ± 18) U/L, the model group (7463 ± 787) U/L had a significant increase in serum amylase.

　　Visual observation of the model group showed significant congestion and edema in the pancreas of rats, increased tension of the pancreatic capsule, varying degrees of bleeding and necrosis, a large amount of bloody ascites in the abdominal cavity, pale yellow saponification spots on the omentum and mesenteric margin, significant edema in the gastrointestinal tract, and paralytic dilation. Bilateral kidneys were significantly enlarged, and the color was dark; Under light microscopy, pancreatic acini are extremely swollen, small blood vessels in the pancreatic parenchyma are dilated and congested, and coagulation necrosis occurs locally. Under high magnification microscopy, a large number of inflammatory cells can be seen infiltrating, necrotic pancreatic cell parenchyma collapses, nuclear fragmentation and dissolution occur, microvascular rupture occurs, causing a large number of red blood cells to overflow into the pancreatic gland stroma.