动物造模,急性坏死性胰腺炎模型,药效评价 z-bio 2024-09-12 964

　　1. Animal modeling material: Adult hybrid dog, 10-15kg, male and female unlimited; Medications: Trypsin, pentobarbital.

　　2. Method of animal modeling: Before the experiment, the animal fasted for 12 hours and couldn't help but water. After anesthesia, the duodenum was found through a midline incision in the upper abdomen. The duodenal wall was cut near the opening of the pancreatic duct, and a thin plastic tube was placed inside the main pancreatic duct. Then, 2-3 ml (0.3 ml/kg) of trypsin and dog's own bile mixture was injected inward, with a formula of 1 ml of bile and 4 ml of pancreatic enzyme solution containing 4 mg of trypsin. The plastic tube was removed after 5 minutes of retention, and the duodenal wall was sutured in a pouch, Close the abdomen.

　　3. Modeling principle: Pancreatobiliary duct obstruction, bile reflux, or duodenal reflux can interfere with the normal function of glandular cells, leading to the activation of trypsinogen by intracellular lysosomal enzymes, leading to pancreatitis.

　　4. Changes after modeling: A significant increase in serum amylase was observed 1 hour after surgery, lasting for 24 hours and reaching its peak at 6 hours; The gross and microscopic pathological changes of the pancreas showed typical necrosis, bleeding, and infiltration of inflammatory cells, indicating that this method can successfully induce a canine model of acute necrotizing pancreatitis. Pathological changes: Extreme swelling of pancreatic tissue is visible to the naked eye, necrosis occurs in the pancreatic parenchyma and under the cell membrane, saponification spots form on the mesentery, and a large amount of bloody ascites and intestinal bloating are seen in the abdominal cavity; Under the light microscope, swelling of pancreatic acinar cells, interstitial edema, bleeding, infiltration of inflammatory cells, microvascular congestion, and a few cases of microthrombosis can be seen. Acinar cell necrosis and adipocyte necrosis.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections. Surgical trauma should be minimized and sterile operations should be strictly carried out.