动物造模,泡球蚴病动物模型 z-bio 2023-11-07 901

　　(1) Method of replication: ① Anesthetize animals infected with echinococcus multilocularis with ether, open the abdominal cavity under sterile conditions, remove the echinococcus multilocularis cyst, rinse with physiological saline, cut the cyst wall, filter 40 mesh, and prepare a physiological saline suspension. Each milliliter of suspension contains 1000U of penicillin. Take 0.1ml and observe the activity of the original scolex under a microscope and count it Inject 500 to 800 E.m. protoscolice into the abdominal cavity of KM mice using a 1ml syringe and a No. 7 needle. ELISA can be used to determine mouse serum antibodies to verify whether the mice are infected.

　　(2) Model characteristics: Injecting E.m. into the original scolex intraperitoneally for 1-3 months, the cyst rapidly increases, and some mice may die due to the cyst being too large or ruptured in the abdominal cavity. Vesicles mainly parasitize in the abdominal cavity, mesentery, spleen, and gastric wall, presenting as yellowish, unevenly sized, and lumpy vesicles. There are fewer liver infections and the volume of the hepatic vesicles is smaller than that of the abdominal cavity, which may be related to intraperitoneal inoculation. Excessive vaccination leads to higher animal mortality rates. This model can cause biological and immunological changes in secondary alveolar echinococcus infection in mice.

　　(3) Different animals in comparative medicine have different susceptibility to infection with echinococcus multilocularis. Different vaccination methods may affect the parasitic site of echinococcus multilocularis. Excessive inoculation of the primary scolex can lead to the death of the model animal. The animal model replicated by this method can be used for drug screening, histopathology, electron microscopy, and other research.