动物造模,过敏反应性结膜炎模型 z-bio 2023-11-07 1006

　　Histamine is an important mediator for rapid allergic reactions and local inflammatory reactions. It can increase the permeability of capillaries, dilate blood vessels, and allow plasma proteins and fluids to enter tissue gaps, causing local edema, thus establishing a model of allergic conjunctivitis.

　　1. Model making method: SD rats, weighing 120-150g, both male and female. Randomly divide rats into groups and administer eye drops, then inject 50 mg/L of histamine phosphate into each eye μ Prepare a conjunctivitis model. After 1 hour, the animals were dissected and the eyelid conjunctiva was weighed to calculate the inhibition rate.

　　2. Observation indicators and analysis of eyelid conjunctiva weight weighing: After 1 hour of inflammation, the animals were dissected and the eyelid conjunctiva was taken for weighing. The weight of the eyelid conjunctiva in the histamine group significantly increased compared to the normal group.