动物造模,乙型肝炎动物模型 z-bio 2023-11-07 824

　　Modeling method: 6-8 week old female BALB/c mice, weighing 14-18g. Grouped according to time points, each group should have at least 3 mice. High pressure water injection method should be used to add a total volume of 0.1ml of compound sodium chloride solution per gram of body weight. Within 5-7 seconds, the solution should be injected into the mice through the tail vein, and each animal should be given 15 doses μ G pHBV1.3 plasmid. On the 1st, 2nd, 3rd, 4th, 7th, 10th, 15th, and 20th days after injection, orbital blood was collected and serum was separated for serological testing. On the 1st, 4th, 7th, 10th, 15th, and 20th day after injection, mice were killed and their livers were taken. Some liver tissues were fixed with 4% neutral formaldehyde for immunohistochemical testing, while the remaining liver tissues were frozen in liquid nitrogen for future use. Real time PCR, ELISA, radioimmunoassay, Southern blotting, Northern blotting, and immunohistochemistry methods were used to detect viral anemia, dynamic changes in HBV antigen expression in serum and liver tissue, HBV transcription and replication in liver tissue, and immune response status in mice.

　　Result: The HBV genome of 1.3copy can be expressed and replicated in mice, and induce specific immune responses in mice. The response mode and HBV clearance process are similar to those of acute HBV infection in humans. This model can be used for research in HBV virology, immunology, and antiviral drug screening.