动物造模,兔结膜炎模型 z-bio 2023-11-06 921

　　Cantharidin tincture has a strong irritant effect, which can stimulate the conjunctiva of rabbit eyes and cause a certain degree of damage, thus replicating the rabbit model of acute irritant conjunctivitis.

　　1. Model making method: New Zealand white rabbits, weighing 2.2-2.5kg, both male and female. Take healthy New Zealand white rabbits with eye diseases excluded, and use a micro injector to drop 50% of cantharidin tincture (10g of cantharidin medicinal material/L) into each rabbit's eye μ l. Once a day, three consecutive times. After the last stimulation, the group was randomly divided according to the experimental design and corresponding treatment was performed before observation.

　　2. Observation indicators and analysis

　　(1) Symptom observation: After animal models of inflammation were established, inflammatory reactions such as congestion and edema of the eyelid and conjunctiva, and increased secretion were observed. The scoring criteria for eye irritation response are normal blood vessels, no edema, and no secretions, with a score of 0; Vascular congestion appears bright red with slight edema and a small amount of secretion, 1 point; Blood vessels are congested and appear dark red, difficult to differentiate, with obvious edema. Some eyelids are everted, and secretions make the eyelids and eyelashes moist or sticky. 2 points; Diffuse congestion appears purple, with edema until the eyelids are almost closed, and secretions make the entire eyelids moist or sticky, with a score of 3.

　　(2) Pathological examination of eyelid and conjunctiva: On the 4th day after administration, animals were euthanized, and one eye conjunctiva tissue was taken from each animal for pathological observation. The model group had conjunctival dilation and congestion, and the histopathological observation score was (1.5 ± 0.55) points.