动物造模,全胰切除糖尿病动物模型 z-bio 2023-11-06 808

　　【 Operation Steps 】 Adult hybrid dog, weighing 10-15kg, with 3% pentobarbital sodium 30mg/kg intramuscular injection and tracheal intubation. One side of the femoral artery and vein were intubated separately to measure arterial pressure and collect blood samples. After anesthesia, intravenous drip of Ringer's solution was administered, and after laparotomy, the pancreas was found. The main supply vessels for the canine pancreas were the splenic artery and the superior and inferior pancreaticoduodenal arteries. The latter forms a anastomotic arch at the upper edge of the pancreas and emits many small blood vessels to supply the duodenum, pancreatic head, and body. The recurrent duodenal artery branches out from the gastroduodenal artery to supply the proximal end of the duodenum. During the surgery, the pancreaticoduodenal anastomosis arch is first dissected. At the lower edge of this anastomosis arch, all small blood vessels originating from the pancreas are ligated and cut off one by one with ophthalmic fine lines, gradually liberating the pancreas. Near the gastroduodenal artery, the anastomotic arch may pass through the pancreatic parenchyma, so it is necessary to remove the upper pancreas as well. Then detach the pancreatic duct from the body of the pancreas and seal it tightly at the distal end to prevent intestinal fistula. Finally, cut off the main supply vessels to minimize their ischemic time as much as possible. Then place the dog in a cage and add pancreatic enzyme tablets to the diet to supplement the insufficient exocrine secretion.

　　[Result analysis] The blood glucose rose to above 11.1 mmol/L within 3 days after total pancreatectomy, and there were symptoms of polydipsia, polyuria, emaciation, and even diabetes ketoacidosis.

　　【 Precautions 】 After the surgery, wrap the animals in a towel to keep them warm, and place them outside the cage until they fully recover from anesthesia before placing them in a dry cage. Within 5 days after the surgery, animals were given physiological saline for drinking, and the suture was removed on the 7th day after the surgery.