动物造模,心律失常 z-bio 2023-10-31 977

　　1. Model making material animal: Rabbit, weighing about 2.5kg, both male and female; Medications: Chloroaldose, Ulatan, Chlorosuccinylcholine; Equipment: Biological function experimental system, stereo locator.

　　2. Method of modeling: Animals were anesthetized with intravenous injection of 0.05g/kg of aldose and 0.5g/kg of urethane, followed by tracheal intubation, artificial respiration, and intravenous injection of 5-10mg/kg of succinylcholine to relax skeletal muscles. Afterwards, half of the amount was supplemented every 0.5-1h to maintain skeletal muscle relaxation, and the left common carotid artery was separated for use. On the animal fixation and stereotaxic device, a stainless steel bipolar stimulation electrode was inserted into the right medial amygdala nucleus (AME) according to the Sawger pattern, coordinates A: 10, R: 2.8, H: 6.5. The electrical stimulation was generated by the 57-6 type pulse stimulator, with a continuous wave frequency of 60Hz, intensity of 20-30V, and stimulation time of 10s. The interval between each stimulation was 10-20 minutes, and the stimulation was repeated 2-4 times, resulting in stable arrhythmia.

　　3. Modeling principle: Electric stimulation of the central nervous system induces arrhythmia and establishes an animal model.

　　4. After modeling, repeated electrical stimulation for 2-4 consecutive times can lead to stable arrhythmia. The myocardial contractility produced by various ectopic rhythms decreases compared to sinus rhythms, and the rhythm affects myocardial contractility. The physiological maximum shortening rate is linearly correlated with the R-R interval of the electrocardiogram. The supraventricular premature contractions produced during sinus bradycardia may exceed the contractile force of the preceding sinus rhythm.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections. Surgical trauma should be minimized and sterile operations should be strictly carried out.