动物造模,病毒性肺热证动物模型 z-bio 2023-10-23 963

　　(1) Reproduction method: Adult rabbits were used to observe their basal body temperature and conjunctival microcirculation blood flow before the experiment. Blood samples were collected from the heart to detect their whole blood viscosity, plasma viscosity, specific antibody titer, and cellular immune function. After 24 hours, the rabbit was fixed on the rabbit platform and in a naturally conscious state, the Sendai virus stock solution (Fushimi, hemagglutination value 1:521) was extracted. It was injected into the trachea through the skin under the cricoid cartilage at a dose of 0.6 ml/kg body weight. Before and 24 hours after modeling, check the microcirculation of the conjunctiva and observe the blood flow pattern and lung X-ray. On the 4th day after modeling, blood was collected from the heart to detect corresponding indicators, and lung tissue homogenate was taken for lipid peroxide detection and histopathological examination.

　　(2) Model characteristics: After the animal is infected with the virus, its body temperature starts to rise at 1 hour, reaches its peak at 10 hours, and can be maintained for more than 58 hours. The animal exhibits strong fever, irritability, shortness of breath, thirst and liking to drink, reduced eating, redness and fever in the ears, dry tongue, red tongue, significantly reduced urination, and dry stool. After 2 days, the chest X-ray showed an increase in lung markings, and after 3 days, spot and patchy shadows were visible; The whole blood viscosity, plasma viscosity, lung tissue homogenate lipid peroxide (LPO) concentration, and serum specific antibody levels were significantly increased; The activity of plasma superoxide dismutase (SOD), C3b receptor rosette rate, and immune complex rosette rate were significantly reduced. On general examination, the lungs of the animals were significantly congested, and dark red lesions with varying levels of light, heavy, and air were visible in patchy or patchy shapes. Some had a large area that could affect the entire lung lobe. The diseased lung lobe was incised, and red fluid flowed out. Under light microscopy, significant lung lesions were observed in the animals, with local alveolar fusion and interstitial congestion visible. Focal or scattered infiltration of phagocytes and lymphocytes was observed in the alveoli; Local exudation of red blood cells and serous substances, shedding of epithelial cells and phagocytes in small and terminal bronchi, infiltration of lymphocytes in the tube wall and mucosa, infiltration of inflammatory cells around the tube, deposition of hemosiderin, and a few nuclear fragments. The microscopic morphology conforms to the changes of viral pneumonia. After 24 hours of infection with the Sendai virus in animals, significant congestion and distortion of capillaries can be observed, with a slow flow of blood, aggregation of red blood cells, and partial venous disconnection.

　　(3) Comparative medicine lung heat syndrome refers to the excess heat syndrome caused by the accumulation of heat pathogens and the loss of lung ventilation. It is also known as lung fire syndrome or heat pathogen obstructing lung syndrome. Its main clinical features include lung symptoms such as thick cough and wheezing, thick phlegm, or foul smell of cough and phlegm, as well as the syndrome of excess heat in the interior. This model was created using tracheal inoculation method, which is easy to operate and has a high success rate; And it causes minimal damage to modeling animals, which can avoid the impact of injury factors on model replication; Sendai virus is a susceptible virus to rodents and humans, with a natural pathological process that is more in line with clinical practice; Although Sendai virus is an important pathogen of human respiratory infections, its infectivity is generally weak, so experimenters are relatively safe; This model is also an animal model of viral pneumonia, so it can be used for the study of antiviral drugs. In addition, the Sendai virus can also create a mouse lung heat syndrome model.