动物造模,急性心力衰竭模型 z-bio 2023-10-16 845

　　（1） Cat acute heart failure model

　　1. Model making material animal: cat, weighing 2-4kg; Medication: pentobarbital.

　　2. Method of modeling: After anesthesia with pentobarbital, the animal was subjected to thoracotomy and left ventricular catheterization. LV dp/dt max was used as a cardiac contractile index, and a constant infusion of 3% pentobarbital was administered intravenously.

　　3. Modeling principle: Pentobarbital causes acute heart failure in animals.

　　4. Changes after modeling: LV dp/dt max gradually decreases after modeling, and when it drops to 26.66-53.32 kPa/s, there is no upward trend after 5 minutes, which is considered to form an acute heart failure model.

　　（2） Guinea pig acute heart failure model

　　1. Animal model material: guinea pigs, weighing 300-400g; Medication: pentobarbital.

　　2. Method of modeling: After anesthesia with pentobarbital, the animal underwent thoracotomy and left ventricular catheterization. LV dp/dt max was used as a cardiac contractile index, and 1.5% pentobarbital was administered intravenously at a constant rate.

　　3. Modeling principle: Pentobarbital causes acute heart failure in animals.

　　4. Changes after modeling: LV dp/dt max gradually decreases after modeling, and when it drops to 13.33-26.66kPa/s, there is no upward trend after 5 minutes, which is considered as the formation of acute heart failure.

　　（3） A canine acute heart failure model

　　1. Model building material animal: dog; Medication: pentobarbital.

　　2. Method of modeling: After anesthesia with pentobarbital at a dose of 30mg/kg, the animal was subjected to thoracotomy and left ventricular catheterization. LV dp/dt max was used as an indicator of cardiac contractility. Within 2-3 minutes, 20 to 30ml of 3% pentobarbital was slowly injected, causing MAP to decrease to 6.65-8.0kPa and LV dp/dt max to decrease to 25% to 30% of the baseline level. Then, pentobarbital was administered intravenously at a dose of 4-6 mg/min to maintain a stable heart failure state (which can be maintained for several hours).

　　3. Modeling principle: Pentobarbital causes acute heart failure in animals.

　　4. Changes after modeling: After modeling, LV dp/dt max gradually decreased to 25% to 30% of the baseline level, resulting in acute heart failure.