动物造模,缺血性心力衰竭模型 z-bio 2023-10-13 855

　　1. Animal modeling materials: Wuzhishan miniature pig, 8-11 months old, male and female, weighing 20-30kg; Medication: Microsphere suspension (composite plastic microspheres are 99% polystyrene+1% ethylenebenzene, with a diameter of approximately 100 μ M (78-112 μ m) The density is 0.18g/ml. 1ml microsphere suspension contains approximately 1 × 100000 plastic microspheres).

　　2. The modeling method adopts a self control method, using cardiac ultrasound, 4F left ventricular contrast catheter, and 5F four chamber thermosensitive balloon floating catheter to monitor and record the changes in cardiac function and hemodynamic parameters before and 2 months after the perfusion of plastic microspheres in the superselected LAD, and to monitor the changes in the levels of cardiac muscle injury markers. The model is successfully established with left ventricular end diastolic pressure (LVEDP)>18mmHg and a decrease of over 30% in cardiac output (CO).

　　Pre operative preparation and anesthesia: administer 1mg of atropine and 10-15mg/kg of ketamine. After inducing anesthesia with intramuscular injection of body weight, intravenous anesthesia with 3% pentobarbital was performed. During the operation, an additional 3-5ml/dose of intravenous anesthesia was added according to the depth of anesthesia, to avoid respiratory depression as much as possible. After satisfactory anesthesia, a 4F arterial sheath was inserted through the right femoral artery using the Seldinger method. According to the standard projection angle, a 4F Pigtail catheter was first used for left ventricular angiography, and LVSP and LVEDP were recorded; Left and right coronary angiography were performed using 4FJL3.5 and JR4.0 coronary artery catheters, and then left coronary artery LAD was superselected using catheter shaping technology. 1ml of plastic microsphere suspension was slowly injected into the LAD using a self guided catheter in stages, (1.0 × 100000 pieces/ml), with an injection time of 60 seconds and an interval of 10 minutes. After each injection, observe the changes in LAD blood flow through angiography and monitor LVEDP until thrombolysis in myocardial infarction (TIMI) blood flow ≤ level 2 and LVEDP>2.0kPa. At the same time, monitor the changes in electrocardiogram and blood pressure. After the LVEDP stabilizes between 2.0 and 2.40kPa, ligate the blood vessels and apply pressure to bandage them. Postoperative limitation of activity. And 4.8 million U of penicilliosol was given twice a day for intramuscular injection for a total of 3 days.

　　3. The principle of modeling is to use the experimental method of fractional anterior descending coronary artery (LAD) microsphere perfusion to cause ischemic necrosis of the anterior wall myocardium in pigs, gradually leading to heart failure.

　　After 2 months of modeling, the success rate of the model was 66.7%. After microsphere injection, coronary angiography showed a decrease in LAD blood flow, and TIMI blood flow was grade 2. Successful modeling of small pigs showed varying degrees of decreased appetite, decreased reactivity, decreased activity, shortness of breath, and moist rales in both lungs, with some small pigs experiencing limb edema. The average weight before model production was (23.7 ± 6.5) kg, and after successful model production, it was (26.6 ± 7.1) kg.

　　After injection of plastic microspheres into LAD, ECG immediately showed extensive ST in the chest lead