动物造模,动脉粥样硬化模型 z-bio 2023-10-12 790

　　1. Model making material animal: quail, male, weighing about 100g; Medication: High fat feed (basic feed with a mass fraction of 1% cholesterol, 14% lard, and 6% soybean oil).

　　2. Modeling method: The normal control group was fed with basic feed every day, while the high-fat model group was fed with high-fat feed for 15 consecutive days; At the same time as feeding high fat feed, different medication treatments were given. The normal control group was given an equal volume mass fraction of 0.5% CMC by gavage; The high-fat model group was given an equal volume mass fraction of 0.5% CMC by gavage, while the positive drug lovastatin group was 7.48mg/kg; The high, medium, and low dose groups of Yizhikang were given 360mg/kg (containing 2.94mg of lovastatin), 180mg/kg (containing 1.47mg of lovastatin), and 90mg/kg (containing 0.74mg of lovastatin) by gavage once a day for 15 consecutive days.

　　3. The modeling principle is to feed animals with high-fat and high cholesterol feed, which will cause atherosclerosis in animals in a short time.

　　4. Changes after modeling: The model group had disorder in the liver cell cord, with lipid droplets of varying sizes appearing in the liver cytoplasm, some fused into larger vacuoles, and most of the fat showed severe degeneration.

　　The arrangement of myocardial fibers in the model group is relatively neat, with fat infiltration and slight steatosis occurring between muscle fibers. Some myocardial cells undergo granular degeneration, and there is a significant increase in epicardial adipose tissue.

　　In the model group of hematoxylin HE staining of aorta, the intima was significantly thickened, a large number of myogenic and macrophage derived foam cells were accumulated under the endothelium, the inner elastic membrane was broken, the smooth muscle of the middle membrane near the intima was irregularly arranged, and the connective tissue between muscle fibers was increased. The endothelial cells in the control group were arranged neatly, with no thickening of the endometrium and smooth muscle of the middle membrane arranged neatly. There was no increase in adipose tissue between the smooth muscle of the middle membrane near the endometrium.

　　The grading of coronary artery lesions is as follows: in the blank control group, the red stained area accounts for more than 10%, and the plaque lesion grading is Grade I; Model group: Red stained areas account for 90% to 100%, and plaque lesions are classified as Grade IV.