动物造模,创伤性脑水肿模型 z-bio 2023-10-11 925

　　1. Animal modeling materials: Healthy large eared white rabbits, weighing 2.5-3.0kg, regardless of gender; Medication: pentobarbital, 10% glucose.

　　2. Modeling method: The animals in the modeling group were anesthetized intraperitoneally with freshly prepared 3% pentobarbital at a dose of 45mg/kg, lying prone, with the head fixed and the head incised, resulting in a left top 1.5cm × A 1.5cm bone window and an impact pad were placed, and a 50g heavy hitting cone was vertically dropped from a height of 30cm to injure the animal while maintaining the integrity of the dura mater. A brain temperature probe was placed outside the dura mater at the edge of the bone window and fixed, the scalp was sutured, and an anal temperature probe was also placed. After injury, the animals were infused with 10% glucose physiological saline intravenously at a rate of 10ml/(kg · h) to maintain their physiological requirements. During the experiment, anesthesia was added to maintain the anesthesia state.

　　3. Modeling principle: Trauma leads to brain edema in animals.

　　4. Changes after modeling: The water content of the brain tissue in the modeling group significantly increased, leading to brain edema.