动物造模,兔肺部白色念珠菌感染模型,药效评价 z-bio 2024-05-23 1152

　　(1) Method of modeling: 36 New Zealand white rabbits with large ears, 18 in the experimental group and 18 in the control group. On the first to fifth days of the experiment, 440 mg/m2 of cytarabine was injected into the ear vein daily, and the immunosuppressive state was maintained every other day after 6 days. Starting from the 4th day, all rabbits were given intravenous injections of vancomycin 15mg/kg and ceftazidine 150mg/kg, once a day; Gentamicin 5mg/kg intravenous injection, once every other day to prevent bacterial infection. Inoculate 0.2ml of Candida albicans suspension (concentration 5) through percutaneous tracheal puncture on the 6th day × Inject a suspension of 10 to the 8th power/ml, and inject an equal amount of physiological saline into the control group. On the 1st to 6th days after 24 hours of vaccination, the animals were euthanized in the designed order, and lung tissue was taken to observe pathological changes; Ligate the left and right main bronchi at the distal end of the carina, repeatedly inject and aspirate 1ml of sterile physiological saline twice, and rinse the rinse solution into sterile test tubes for future use.

　　(2) Result: The blood granulocyte count in the rabbit ear vein significantly decreased after injection of immunosuppressants; There were varying degrees of inflammatory changes in the lungs of 16 rabbits (16/18) in the experimental group, and spores and hyphae were observed at all lesion sites. The wall of the bronchioles thickens, a large number of fungal spores infiltrate and grow, and the lymphocytes on the tube wall proliferate. The surrounding alveolar wall thickens and there is a small amount of protein extract in the cavity. The local alveolar structure disappears, presenting uniform and uniform necrosis material filling and consolidation, with a large number of hyphae and spores visible.