[Animal Modeling] - Toxic Shock Model

动物造模,中毒性休克模型

z-bio

2023-10-11

851

　　Method 1

　　1. Animal modeling materials: rats or dogs; Drug: Escherichia coli lipopolysaccharide.

　　2. Method of modeling: Rats were injected intravenously with Escherichia coli lipopolysaccharide (O55 class B lipopolysaccharide) 4mg/kg. Alternatively, E. coli lipopolysaccharide O111 B 5mg/kg is administered intravenously to dogs.

　　3. Modeling principle: Lipopolysaccharides cause shock in animals.

　　4. Changes after modeling: 45 minutes and 60 minutes after injection, the blood pressure of rats decreased from (14.60 ± 1.00) kPa to (11.47 ± 1.83) and (9.64 ± 2.21) kPa, respectively. After 4 minutes of injection, the blood pressure of the dog decreased to the lowest level, rebounded after a few minutes, decreased again after 30 minutes, and decreased by 48% after 60 minutes.

　　5. Precautions: Bacteria and lipopolysaccharides of different genera and batches have a significant impact on the experimental results. Therefore, lipopolysaccharides of the same genus, serotype, and batch number should be selected for the experiment.

　　Method 2

　　1. Model material animal: New Zealand white rabbit, male and female, weighing 2.0-2.5kg; Medication: pentobarbital, lipopolysaccharide (LPS).

　　2. Modeling method: Fasting for 8 hours and drinking for 4 hours before the experiment. 3% pentobarbital was injected into the rabbit ear vein at a dose of 1ml/kg for anesthesia, and the animal was fixed in a supine position. A monitor was placed in the right femoral artery to monitor blood pressure in real-time and collect arterial blood. Right femoral vein catheterization for medication and infusion. Right external jugular vein catheterization for blood collection. Measure the basic value after stabilizing for 30 minutes. At 600 μ Continuous injection of LPS at g/kg, with each milligram of LPS dissolved in 10ml of physiological saline. After 30 minutes of injection, shock occurs when the mean arterial pressure (MAP) is below 7.98kPa.

　　3. Modeling principle: Lipopolysaccharides cause shock in animals.

　　4. Changes after modeling: Model group animals were injected with lipopolysaccharide 600 μ After g/kg, blood pressure began to decrease, briefly rebounded, and then decreased again. After 2 hours, blood pressure dropped below 7.98kPa, accompanied by symptoms such as restlessness, ear cooling, and cyanosis, indicating the successful replication of the model. Three animals in this group died within 8 hours, all of which developed arrhythmias; All deaths within 24 hours.