动物造模,兔肺部烟曲霉菌感染动物模型 z-bio 2023-10-11 880

　　(1) Method of modeling: New Zealand large eared white rabbits were injected daily with 440mg/square meter of cytarabine via ear vein from the 1st to 5th day of the experiment, and maintained a low immune state once every other day after 6 days. Starting from the 4th day, both groups were given intravenous injections of vancomycin 15mg/kg and ceftazidime 150mg/kg once a day; Gentamicin 5mg/kg intravenous injection, once every other day. On the second day of the experiment, intratracheal inoculation was carried out, and after anesthesia, 0.4ml of Aspergillus solution (concentration 5) was injected into the trachea × 10 to the 7th power/ml). The control group was injected with 0.4ml of physiological saline into the trachea. Perform lung CT scans on all surviving New Zealand white rabbits 1 day before vaccination, 2 days, 4 days, 6 days, 8 days, 10 days, 12 days, 14 days, 16 days, and 18 days after vaccination. After each CT examination, two experimental rabbits with abnormalities were euthanized and specimens were taken for pathological and fungal culture. At the end of the experiment, collect pathological and fungal culture results and summarize all CT findings.

　　(2) Result: 17 New Zealand white rabbits were successfully inoculated with Aspergillus fumigatus, 6 rabbits found lung abnormalities on the 2nd day of inoculation, 4 rabbits found abnormalities on the 4th day, 3 rabbits found abnormalities on the 6th day, 2 rabbits found abnormalities on the 8th day, 1 rabbit found abnormalities on the 12th day, and 1 rabbit found abnormalities on the 18th day. During the experiment, 10 experimental rabbits died spontaneously. The pathological changes manifest as pulmonary congestion, bleeding, interstitial hyperplasia, infiltration of inflammatory cells, tissue necrosis, and abscess formation.