动物造模,实验性脑血栓模型 z-bio 2023-10-11 1023

　　1. Model material animal: SD rat, male, weighing (350 ± 30) g; Medication: 3% pentobarbital, Evans blue (0.2%), thrombin; Instrument: Physiological multi-channel instrument.

　　2. Method of modeling: Rats were anesthetized intraperitoneally with 3% pentobarbital 30mg/kg, and the trachea was separated for tracheal intubation; Separate the right common carotid artery and separate the right external carotid artery forward along the common carotid artery; Ligate the external carotid artery and the centripetal end of the common carotid artery, inject 0.1ml/100g of thrombus inducer into each animal from the common carotid artery, and then use suture to ligate the needle eye after injection. Inject Evans blue (0.2%) 5ml/kg into the right common carotid artery 5 minutes later; After 5 minutes, the head was quickly severed and executed.

　　3. Modeling principle: Thrombolytic inducers promote the formation of cerebral thrombosis in rats.

　　4. Changes after modeling: After the creation of a cerebral thrombosis model, it can be clearly seen with the naked eye that the right hemisphere is blue stained, with the degree of blue staining at the skull base being the most severe. No blue staining can be seen in the left hemisphere, indicating that the right hemisphere thrombosis model has been successfully manufactured. Compared with the control group, it was found that after the formation of the cerebral thrombosis model, the proportion of right brain wet weight to left brain wet weight increased.