动物造模,兔双侧股动脉急性血栓模型 z-bio 2023-10-09 967

　　1. Animal modeling material: New Zealand rabbits, male and female unlimited; Medication: Anesthetics; Instrument: Doppler blood flow meter.

　　2. Method of modeling: After recording the basic blood flow of the femoral artery using a Doppler flow meter, the distal femoral artery segment was repeatedly pinched and pressed 10 times with a rubber hemostatic forceps, each time for 5 seconds, causing damage to the intima. First, clamp the blood vessels with arterial clamps at the distal end, and clamp the proximal end at an interval of 1cm to form a thrombus rich in red blood cells. Release the arterial clamps at both ends after 30 minutes. Record the value of femoral artery blood flow. The blood flow value of the femoral artery is basically zero, with a frequency of 13.0MHz. No blood flow or vascular ultrasound microbubble imaging was detected, confirming that there were no microbubbles passing through, indicating that the skin was temporarily sutured after successful thrombus modeling.

　　3. Modeling principle: Damage to vascular endothelial cells can lead to thrombosis.

　　4. Changes after modeling: After animal thrombosis, the Doppler blood flow signal in the blood vessels was interrupted. After intravenous injection of microbubble contrast agent, only a small amount of microbubble contrast agent was seen passing through, indicating the success of the acute arterial thrombosis model.