动物造模,异种移植银屑病小鼠模型 z-bio 2023-10-09 980

　　1. Nude mouse model: The mice used in this model have no thymus and cellular immune deficiency, which does not cause immune rejection. If psoriasis skin lesions are transplanted into nude mice, without further intervention, the skin lesions can last for more than 2 months.

　　2. After obtaining the patient's consent, the SCID mouse model is first taken from its waist or buttocks (using 6cm) × 2cm × 0.05cm corneal knife, including both epidermis and dermis.

　　Construction and tissue treatment of human skin/SCID mouse chimeras: Human skin transplantation was performed on SCID mice (18-25g, 4-6 weeks old), followed by transplantation (1.5cm) × 1.5cm × 0.05cm) was sutured onto both sides of the mouse body using 4-0 absorbable sutures, and finally covered with a dressing. The dressing should be changed every 2 days, and the animals should always be kept in a sterile environment. After 3 weeks, autologous T cells were injected into the dermis of the transplanted skin. Most of the transplanted skin exhibited a psoriasis phenotype, such as disappearance of the granular layer, extension of the skin process, infiltration of T lymphocytes, and proliferation of dermal blood vessels.

　　3. AGR129 mouse model

　　(1) The characteristics of this model are: defects in IFN - Ⅰ and Ⅱ receptors and lack of RAG-2 gene, resulting in a lack of T and B cells, immature NK cells, and incomplete cytotoxic function.

　　(2) The construction of this model: Ensure that patients are prohibited from local/systemic medication treatment within four weeks before the start of the study, and that mice are always operated in a sterile environment after the start of the study. Initially, asymptomatic skin was taken from the waist or buttocks of patients with plaque psoriasis using a corneal knife, and then the skin was transplanted onto the back of mice, sealed and fixed with absorbable tissue. Psoriasis like skin lesions began to appear in the graft at week 4, and developed completely by weeks 6-8. In the final 31 AGR129 mouse grafts, 28 developed psoriasis, and the skin lesions were clinically and histopathologically identical to those of the original psoriasis patient.