动物造模,溃疡性结肠炎 z-bio 2023-10-09 799

　　1. Animal modeling materials: 5-6 month old rats, male and female, weighing 250-400g; Medication: pentobarbital.

　　2. Method of modeling: Rats were anesthetized intraperitoneally with pentobarbital, and three pregnant rats from 14 to 18 days of pregnancy were taken out. The colon of the fetal rats was euthanized and taken out, with a length of 3-4 cm. They were buried under the capsule of the right kidney of the adult rat using aseptic techniques, and were raised normally after surgery.

　　3. The principle of modeling is to use the fetal mouse colon as an antigen and transplant it under the renal capsule of adult mice of the same line, resulting in a typical cellular immune response, which is the host anti graft response.

　　4. Changes after modeling: The colon mucosa of rats in the modeling group showed a large number of lymphocyte infiltration and lymphatic follicles, local mucosal necrosis and detachment, forming ulcers or erosive lesions. On 7 days, lymphocytes and monocytes infiltrated the colon mucosa layer and submucosa, with some mucosa missing and shedding. There was a large amount of mucus secretion in the intestinal cavity, and on 21 days, the mucosal glands were severely atrophic and degenerated, with fibrous connective tissue hyperplasia.

　　5. Notes: The technical key of this model is that the surgery should be performed aseptically to avoid animal death caused by surgical infection as much as possible. This experiment has a long cycle, high technical requirements, low success rate, and symptoms similar to clinical symptoms, indicating chronic inflammation.