动物造模,肺气虚证动物模型 z-bio 2023-10-09 807

　　(1) Reproduction method: Adult rats were injected with lipopolysaccharide (LPS) 200 into the trachea on the 1st and 14th days, respectively μ G/200 μ L. On the 2nd to 13th and 15th to 28th mornings, smoke 5% cigarette smoke in a 72L fumigation box for 0.5 hours. Observe the general condition, respiratory rate, and body weight of the animals. After modeling, execute the animals and take lung tissue for pathological examination.

　　(2) Model characteristics: reduced animal activity, hunched back, reduced weight gain, shortness of breath, frequent coughing, furrowing, reduced appetite, delayed movement, reduced weight gain, and increased respiratory frequency. The lungs of the animal are in a swollen state, with an increase in volume and slightly uneven surface, with visible small vesicular protrusions; Partial detachment of tracheal mucosal epithelium, proliferation and hypertrophy of goblet cells and glands, and infiltration of inflammatory cells in the tube wall; The wrinkled walls of the bronchial mucosa increase and become longer; A large number of neutrophils can be seen in the lumen of the small bronchi; There is obvious infiltration of inflammatory cells in the tube wall and its surroundings; Distal stenosis of the terminal bronchioles, cystic dilation of the respiratory bronchioles and alveolar ducts, enlargement of the alveoli, thinning of the alveolar walls, presenting as central lobular emphysema; Some peripheral lung tissues have enlarged alveoli, presenting as alveolar emphysema; The cytoplasm of rat bronchial ciliated columnar epithelial cells swells, cilia decrease and become shorter, and some cilia fall off; Type II alveolar epithelial cells show depletion of lamellar bodies and vacuolar degeneration of mitochondria; Macrophages and a few red blood cells can be seen in the alveolar cavity.

　　(3) The symptoms of the animal model in comparative medicine are consistent with the manifestations of lung qi deficiency syndrome. A lung qi deficiency syndrome model combining disease and syndrome was created using a composite factor method, which has the characteristics of short time, easy replication, and strong operability, and is also in line with the actual clinical situation.