动物造模,腺胃黏膜肠上皮化生模型 z-bio 2023-10-09 880

　　1. Animal model material: Wistar rats, male, weighing 120-140g; Medications: Methylnitrosoguanidine, Ranitidine.

　　2. Modeling method: The rats in the modeling group were added MNNG to their drinking water at a concentration of 60mg/L, allowing the animals to freely drink. They ate a powder feed containing 0.03% ranitidine, and the administration time was 20 weeks. The normal control group did not undergo special treatment.

　　3. Modeling principle: Alkyl nitroso nitroguanidine can induce intestinal metaplasia of animal gastric mucosa.

　　4. After 7 weeks of administration, changes were observed under light microscopy. Scattered erosion points were observed in the gastric mucosa, and intestinal metaplasia appeared as early as the 9th week. On tissue sections before the 12th week, metaplastic glands were limited to the glandular neck, characterized by the appearance of goblet cells. At the 16th week, metaplastic glands can be seen extending from the neck of the gland to the bottom of the mucosa. After 20 weeks, intestinal metaplasia can be seen in different parts of the gastric mucosa. Over time, the degree and scope of intestinal metaplasia have increased.