动物造模,肝气郁结证动物模型 z-bio 2023-10-09 943

　　(1) Reproduction method: Adult rats were used to clamp the tail of one of the rats with a hemostatic forceps wrapped in gauze at the tip, causing it to tear and beat with other rats, indirectly provoking the entire cage of rats. Indirect provoking rats were used as experimental mice, with each stimulation lasting for 30 minutes, with no skin breaking or bleeding. Stimulation was performed every 3 hours, 4 times a day, and the general condition of the animals was observed for 2 days. After the completion of modeling, the animal's gastrointestinal motility was detected, blood was taken for biochemical examination, and platelets were observed under electron microscopy.

　　(2) Model characteristics: When clamping the tail of the animal, the whole cage animal becomes angry. As the number of stimuli increases, the biting becomes more severe. After 3 days, the intense biting between animals weakens, the amount of water and food consumed decreases, the body becomes tired, the hair becomes dark and yellow, and the weight decreases; The platelet aggregation rate, whole blood viscosity, and plasma specific viscosity increase, the calcium recovery time prolongs, the hematocrit decreases, and the erythrocyte sedimentation rate accelerates; Under electron microscopy, platelets can be seen to have dendritic protrusions and many microtubules, with the majority being adhesive aggregates; The gastric residual rate increases, the small intestine propulsion rate decreases, and the plasma SS level increases.

　　(3) Comparative medicine refers to the animal model of liver qi stagnation syndrome caused by bandage restraint. In addition, the strength of the tail clamping method is related to the occurrence of symptoms. Excessive strength may lead to blood stasis syndrome. At the same time, tearing and biting between animals can cause skin damage, bleeding, erosion, and scabbing, which is also a manifestation of local blood stasis syndrome. Therefore, attention should be paid when evaluating symptoms.