动物造模,胃炎模型 z-bio 2023-10-09 844

　　1. Model making material animal: China No.1 small pig; Medication: Helicobacter pylori strain.

　　2. Moulding method: After the full-term pregnant sows show signs of delivery, a small amount of ketamine is used for anesthesia, cleaning, and strict abdominal disinfection. The fetuses are taken from the operating room by laparotomy, and the piglets are placed in a sterile isolator for feeding. Sterilized milk is given, and they are fed freely. After 10 days, they are divided into groups. Model group animals were given 12.5mg of indomethacin once a day for 3 days. The control group did not undergo any treatment.

　　On the 14th day, the molding team administered 2ml of Hp (1 × 10 to the 9th power CFU/ml), once per 3 days, administered orally for a total of 3 times. The control group was given an equal amount of physiological saline.

　　3. Modeling principle: Helicobacter pylori causes animal related gastritis.

　　4. Changes visible to the naked eye after modeling: After the piglet was euthanized, the stomach was taken out and cut along the greater curvature of the stomach, flattened, and the mucosal changes were examined. Most animals in the modeling group showed slight thickening and edema of the gastric mucosa on the surface, accompanied by superficial erosion. The control group showed no pathological changes in the mucosa of the esophagus, stomach, and duodenum.

　　HE staining tissue sections: In the esophageal, gastric, and duodenal mucosa of the model group animals, there were varying degrees of superficial mucosal erosion, inflammatory exudation, infiltration of inflammatory cells in the lamina propria, and some lymphoid follicular hyperplasia. These pathological changes can confirm that the piglets infected with Hp have undergone pathological changes of chronic active gastritis. The control group showed no inflammatory changes in the mucosa.

　　Urease test: The esophagus, gastric body, gastric antrum, duodenum, small intestine, and large intestine mucosa of the model group piglets all showed positive reactions. The control group's urease test showed a negative reaction.

　　Warthin Starry silver staining: In the modeling group, there were a large number of rod-shaped or s-shaped Hp bacteria stained black on the surface of the gastric body and gastric antrum mucosa of piglets, and some lesions showed accumulated Hp. Clear Hp stained black brown can also be seen in the esophageal and duodenal mucosa.

　　Hp bacteriological culture: The model group of piglets cultured Hp bacteria, which had a white and transparent colony with a diameter of about 1mm. After smearing, a large number of Hp bacteria were visible, and urease and Hp-PCR tests showed positive reactions, which were basically consistent with the results of urease tests.