动物造模,血管外膜炎症模型 z-bio 2023-09-27 830

　　1. Model material animal: New Zealand large eared white rabbit, male and female, weighing 1.5-2.0kg.

　　2. Method of modeling: Preoperative feeding with a high-fat diet (regular basic feed+7% peanut oil+1.5% cholesterol) for two weeks. After anesthesia with 30mg/kg ketamine+5mg/kg, the animals were subjected to routine disinfection. The skin at the inguinal area was cut open to expose the femoral artery, and a polyethylene sleeve (1.5cm in length, 1.5mm2 in inner diameter, and 2.0mm2 in outer diameter) was placed over the femoral artery. A medical cotton thread (1.5cm in length) soaked overnight with 6mg/L lipopolysaccharide physiological saline was placed between the sleeve and the artery, allowing the cotton thread to fully contact the outer membrane of the artery. The opposite side was treated with physiological saline instead of 6mg/L lipopolysaccharide physiological saline, All animals were still fed a high-fat diet after surgery.

　　3. Comparison of biochemical changes after modeling with the control group's vascular intimal area: The vascular intimal area was (0.71 ± 0.05) mm2 at 0 weeks after modeling, and (0.75 ± 0.15) mm2 at 2 weeks after modeling.

　　4. Pathological changes after modeling: No inflammatory cell infiltration was observed in the vascular wall 0 days after modeling; On the 3rd day after surgery, significant inflammatory cell infiltration was observed on the outer membrane surface, and inflammatory cell adhesion was also observed on the vascular endothelium; At 2 weeks after operation, inflammatory cells in the adventitia were still seen infiltrating, mostly monocyte macrophages, and a small number of foreign body macrophages. In addition, smooth muscle cells in the middle membrane of the blood vessels showed foam, and intimal hyperplasia was seen. Sometimes, plaque appeared in the intima and protruded into the lumen.