动物造模,兔血栓模型 z-bio 2023-09-27 728

　　1. Animal modeling material: Healthy male New Zealand rabbits, weighing (1.91 ± 0.18) kg; Medications: cholesterol, angiotensin II, Russell viper venom.

　　2. Modeling method: The model group animals were fed with cholesterol (1g/d per animal) and standard pellet feed (100g/d per animal) for 18 weeks. The control group animals were fed standard pellet feed (100g/d per animal) for 18 weeks. At the end of the 18th week, puncture the ear vein or central ear vein and place an indwelling needle. Intraperitoneal injection of RVV 150 μ G/kg, inject AT Ⅱ 30 intravenously from the indwelling needle after 0.5 hours μ G/kg, establish a thrombus model.

　　3. The principle of modeling is that unstable atherosclerotic plaque rupture and thrombosis lead to partial or complete blockage of vascular lumen.

　　4. Changes in HE staining after modeling: It can be seen that the thickness of atherosclerotic plaque on the arterial wall of cholesterol fed model group rabbits is 0.5~1.5 times that of the arterial wall, the plaque center is a large number of unstained lipids, and there are large foam cells infiltrating the plaque. Thrombosis HE staining showed the intima being lifted and mural thrombus formation, but no thrombus was directly connected to the plaque. The thrombus formation rate was 60%. Under high-power microscopy, the thrombus components in the model group were mainly composed of a large number of platelets, which were stained in a homogenous state, and cellulose, red blood cells, and white blood cells were also visible. Irregular distribution of yellow brown hemosiderin particles can be observed in the thrombus.