动物造模,诱发性银屑病动物模型 z-bio 2023-09-27 895

　　1. Application of propranolol to create a guinea pig ear psoriasis model. This model is commonly used in China. Huang et al. applied 5% propranolol to the ears of guinea pigs (350-400g) (0.3g of drug per cm2) twice a day for 2 weeks, while using 1% dinitrochlorobenzene (DNCB) and 1% croton oil castor oil as the control group. The results confirmed that psoriasis like skin lesions appeared in the ears of guinea pigs in the propranolol group, and this skin lesion was caused by the unique pharmacological effects of propranolol, rather than irritating dermatitis.

　　2. Jensen et al. selected hairless rats of no more than 3 weeks old, regardless of gender, with an average body weight of 47.5g at the beginning of the experiment. Each rat is raised separately, eating and drinking freely, and given 12 hours of sunlight daily, with a temperature of 22-26 ℃ and a humidity of about 30%. The food used for feeding lacks essential fatty acid (EFAD), and its formula is 20% mass fraction of edible casein (containing 0.01% linoleic acid), 70% sucrose, 5% cellulose, and 5% vitamins and inorganic salts. The construction of the model was evaluated based on animal weight, water consumption, transdermal water loss, clinical skin manifestations, histopathology of the epidermis, and fatty acid content in serum and skin. The results showed that the model could be successfully established within 8 weeks.

　　In addition, ultraviolet radiation can stimulate the epidermal cells of nude mice to accelerate renewal, resulting in excessive keratinization and simulating psoriasis.