动物造模,体内血栓形成动物模型 z-bio 2023-09-26 771

　　1. Animal modeling material: Wistar rats, male and female, weighing 200-250g; Medication: Ethyl carbamate, heparin injection; Equipment: Disposable intravenous indwelling needle, BL-420 biological function experimental system.

　　2. Modeling method: Divide the rats into two groups. One group is the physiological saline model group, and the other group is the heparin model group. Both groups of animals were anesthetized with 3% ethyl carbamate (5ml/kg) and underwent tracheal intubation. The right common carotid artery was separated and arterial intubation was performed using a venous indwelling needle. The intubation was connected to the BL-420 system through a pressure transducer, and the blood pressure curve was recorded. The saline model group was injected with saline through the femoral vein, while the heparin model group was injected with heparin (1200U/kg). After 5 minutes of administration, a self-made hook shaped protective electrode was used to contact the common carotid artery 0.5cm below the arterial cannula (near the cardiac end), and the artery was moderately lifted to block blood flow, causing the blood pressure curve to disappear and become straight. Activate the stimulator and continuously stimulate the arteries with direct current. Set the stimulus intensity to 3mA, stimulus frequency to 10Hz, wave width to 5.0ms, and delay to 0.1ms. Observe the changes in arterial color at the stimulation site during the stimulation process. After the color turns purple, relax the electrode every 20 seconds, but the electrode still comes into contact with the artery. Observe if there is a pressure curve. If a pressure curve appears, it indicates that the thrombus has not completely blocked the artery, and continue to stimulate; If no pressure curve appears, stop stimulation and record the time from the start of stimulation to the disappearance of the pressure curve, which is the time of thrombus blockage.

　　3. Modeling principle: Damage to vascular endothelial cells can lead to thrombosis.

　　4. Changes after modeling: Both model groups showed arterial embolism, with an average thrombus blockage time of (15.6 ± 5.3) minutes in the saline model group and (90.2 ± 12.5) minutes in the heparin model group.

　　5. Precautions: During the stimulation of the common carotid artery, an electrode should be used to moderately lift the artery, making it flat and blocking blood flow. The purpose is to prevent newly formed blood clots from being dispersed by the blood flow and not easily causing complete blockage.