动物造模,烟熏法肺气虚证 z-bio 2023-09-26 978

　　(1) Reproduction method: Adult rats or rabbits were placed in a smoke chamber with a volume of 1m3. 30-50g of shavings, sawdust, and tobacco were ignited and smoked once a day for 30 minutes each time. The smoking time of the rabbits was doubled, and their symptoms and signs were observed and recorded daily. After 55-75 days, immunological, hemodynamic, pulmonary blood flow, and respiratory dynamics indicators were measured, and their pathological morphological changes were dissected and observed.

　　(2) Model characteristics: The animal lost weight, and after two weeks, coughing and sparse saliva appeared. The rabbit occasionally coughed. After six weeks, the rat experienced coughing, wheezing, shortness of breath, and the rabbit's cough worsened. Mucous secretions flowed out of the mouth and nose, and symptoms and signs such as mental fatigue, slow movement, curling up and immobility, excessive phlegm, insufficient appetite, thin and loose stools, shedding of body hair, and blue tongue color appeared successively. The decrease in OD value slowed down after injection of ink in the carbon particle clearance test of model animals; The neutrophil phagocytic rate, phagocytic index, lymphocyte conversion rate, macrophage rate, red blood cell G3b receptor rosette rate, serum IgG, and IgA in mucus secretion decreased.

　　(3) Comparative medicine lung qi deficiency syndrome refers to a syndrome characterized by insufficient lung qi and weakened main qi and external defense functions. The main clinical features are weak cough and asthma, clear and thin phlegm, and qi deficiency syndrome. Through synchronous clinical and experimental research, it was found that the immune function of model animals showed significant changes, mainly manifested as a significant decrease in cellular and humoral immune functions. This model is the most commonly used model in the study of lung qi deficiency syndrome. Based on long-term clinical practice and understanding of its pathogenesis, this modeling method is not only in line with the pathological model characteristics of Western medicine's "disease", but also basically in line with the "lung qi deficiency syndrome" of traditional Chinese medicine. It is a "disease syndrome combination" method. The model can be classified as lung qi deficiency and chronic bronchitis animal models with lung qi deficiency.