动物造模,噁唑酮结肠炎 z-bio 2023-09-26 747

　　1. Animal model material: BALC/c mice, 7-8 weeks old, weighing 25-30g; Medications: Oxazolidone, Mizumab, Ethanol.

　　2. Modeling method: Model mice were injected intramuscularly with fast sleeping new anesthesia, and their abdominal skin was shaved (2cm) × 2cm), apply 0.2ml of 3% oxazolone (dissolved in 100% ethanol) to the skin of the model group; Repeat the application once after 1 day. After 5 days, a 2mm diameter silicone tube was inserted into the mouse intestine from the anus to a depth of about 4cm. The model group was injected with 0.15ml of 1% oxazolone (dissolved in 505 ethanol).

　　3. Modeling principle: Oxazolone induces colitis in mice.

　　4. General changes after modeling: Two days after the second application of oxazolone in the model group mice, the skin at the application site showed redness and swelling, and some mice had skin ulceration. The model group mice showed weight loss and diarrhea 24 hours after enema. The diarrhea reached its peak on the 3rd to 4th day, and some mice showed bloody stools with naked eyes. The diarrhea lasted for about 1 week and gradually turned into soft stools. After 2 weeks, the stool characteristics returned to normal.

　　The model group mice showed congestion and edema of the distal colon mucosa 24 hours after enema, with a continuous distribution of lesions. Microscopically, the lesions showed epithelial cell loss, erosion, and shallow ulcer formation, reduced goblet cells, decreased glandular density, and inflammation limited to the mucosa and submucosa. Multiple inflammatory cell infiltration was observed in the lamina propria of the mucosa, with neutrophils infiltrating primarily in the early stage and lymphocytes, monocytes, and plasma cells infiltrating mainly after 1 week, A few neutrophils and eosinophils can be seen; Colitis can last for about 2 weeks.

　　The activity of myeloperoxidase (MPO) in the colon tissue of the model group mice was significantly increased within 1 week (24h, 3rd, and 7th days) after enema compared to the control group. The IL-4 content in the colon tissue of the model group mice was significantly increased compared to the control group within 1 week after enema (on the 24th, 3rd, and 7th days), with TNF- α And interferon- γ (inter feron- γ, IFN- γ) The content is basically normal.