动物造模 z-bio 2023-09-26 761

　　(1) Reproduction method: Adult rats were given a dose of 50% medical ethanol 1ml/100g body weight by gavage on the 1st day, Baoning vinegar 1ml/100g body weight by gavage on the 2nd to 11th day, and 1% aspirin 1ml/100g body weight by gavage on the 12th to 25th day. At the end of the experiment, the counts of red blood cells, white blood cells, and platelets were measured, as well as the rosette rate of red blood cell C3b receptor and the rosette rate of red blood cell C3b receptor immune complex. The animals were decapitated and killed, and the thymus and spleen were taken for histological examination.

　　(2) Model characteristics: animal hair erection, diarrhea, decreased appetite, weight loss, decreased body temperature, decreased spontaneous activity, positive fecal occult blood test, and prolonged clotting time; On autopsy, there is spot or patchy bleeding in the animal's stomach and duodenum; Under the light microscope, it can be seen that the thymic cortex has significantly thinned, the number of cortical lymphocytes has decreased, PAS positive reticulum epithelial cells in the thymic medulla have significantly decreased, and the T and B cell areas of the spleen have significantly shrunk; The white blood cells, red blood cells, platelets, RBC-C3 b receptor rosette rate, and RBC-IC rosette rate decreased.

　　(3) Comparative medical wine is a dry and strong product, vinegar is a souring agent, and wine vinegar can cause spleen deficiency. Spleen deficiency is the foundation of spleen failure to regulate blood, and there should be inducing factors for spleen deficiency bleeding. Among many causes of spleen deficiency bleeding, the dosage of aspirin is low (this dosage alone is not easy to bleed), the model is stable, and the repetition rate is high.