动物造模,溃疡性结肠炎模型 z-bio 2023-09-22 870

　　1. Animal modeling materials: 42 male SD rats, weighing (200 ± 20) g; Medication: 2,4,6-trinitrobenzenesulfonic acid.

　　2. Method of modeling: Fasting for 24 hours on the day before modeling. In the normal group, a polypropylene tube was inserted 8cm into the upper anal segment and 0.85% NaCl 0.6ml was injected at once; Model group: TNB was calculated at 100mg/kg (approximately equal to 0.18ml/100g of TNB original solution) and 0.25ml of 50% ethanol was added. A polypropylene tube was inserted into the upper anal segment for 8cm and mixed reagents were injected at once.

　　3. Modeling principle: The TNB/ethanol modeling method belongs to the semi antigen induced model of the normal immune system. When TNB/ethanol enema is performed, ethanol acts as an organic solvent to dissolve the mucus on the surface of the intestinal mucosa, temporarily disrupting the intestinal mucosal barrier, causing TNB to bind with intestinal tissue proteins to form a complete antigen, leading to delayed allergic reactions of the intestinal mucosal immune system to this antigen and causing damage to the intestinal mucosa.

　　4. General changes after modeling: One week after the completion of modeling, compared with the normal group, the model group animals showed a decrease in food intake, digestive symptoms such as loose feces or mucous bloody stools, as well as systemic changes such as mental fatigue, decreased activity, dull hair color, and weight loss.

　　5. After modeling, the pathological changes were observed with naked eyes. There was no tissue damage, mucosal congestion, edema, or ulcer formation in the normal group; The model group showed multiple ulcers with significant mucosal congestion and edema. After routine hematoxylin eosin staining, under the light microscope, a large number of inflammatory cells infiltrated the submucosa of the model group, ulceration formed, and significant congestion and edema were observed in the submucosa.