动物造模,再生障碍性贫血(放射)血虚证 z-bio 2023-09-14 985

　　(1) Reproduction method: Adult mice, 60 Co after peripheral blood count detection- γ A 3.5Gy full body irradiation with an absorbed dose rate of 1.27Gy · min-1, irradiation time of 161s, and irradiation distance of 4m. Subsequently, peripheral blood samples were continuously tested; After 7 days of modeling, some animals were euthanized and femurs were taken. The production capacity of granulocytes (CFU-GM), erythrocytes (BFU-E, CFU-E), megakaryocytes (CFU meg), and mixed lineages (CFU mix) in mouse bone marrow, as well as the changes in CD34+cells, were measured. The thymus and spleen were weighed.

　　(2) Model characteristics: The total number of white blood cells in the peripheral blood of mice decreased to its lowest point 3 days after irradiation, then briefly rebounded and then slightly decreased. It dropped to its lowest point again 11 days after irradiation, and then recovered; The CFU-GM, CFU-E, BFU-E, CFU-meg, and CFU-mix colonies in the bone marrow were significantly reduced, and the proportion of CD34+cells was significantly reduced; The spleen index and thymus index significantly decreased.

　　(3) 60Co for comparative medicine- γ Irradiation of the entire body of animals can damage bone marrow and affect hematopoietic function. This model is closer to clinical aplastic anemia, but special equipment makes it difficult to control the radiation dose, which is too small to meet the damage requirements, and excessive radiation can lead to animal death. Sometimes, the effects of drugs are difficult to observe.