动物造模,慢性胃、十二指肠溃疡 z-bio 2023-09-13 1212

　　（1） Gastric ulcer model induced by acetic acid immersion method

　　1. Model making material animals: rats, weighing 200-250g; Medications: pentobarbital, acetic acid, iodine alcohol.

　　2. Method of modeling: Fasting for 24 hours, modeling rats were anesthetized by intraperitoneal injection of pentobarbital 30mg/kg, skin was disinfected with iodine, and abdominal surgery was performed. 0.015ml-0.15ml (usually 0.05ml) of 10% -40% acetic acid solution (usually 20%) was injected into the gastric mucosal submucosa through the anterior wall of the gastric antrum; Alternatively, use a cotton swab dipped in 0.1ml of 100% acetic acid to directly immerse the serous surface of the sinus through a circular ring; Or use a special printing clip to add 0.1ml of 100% acetic acid and tightly clamp the stomach wall for about 1 minute. Use filter paper to remove acetic acid attached to the surface of the stomach, partially cover the omentum, suture the abdominal wall, and close the abdominal cavity.

　　3. Modeling principle: Acetic acid causes gastric ulcers in animals.

　　4. Changes after modeling usually result in gastric ulcer formation 3-5 days after surgery. Gastric ulcers induced by this method generally heal on their own after about 60 days, but there are also some cases where larger ulcers can still be seen after 200 days.

　　5. Precautions: If the concentration of acetic acid is too high or the contact time is long, it can cause ulcers or even perforation.

　　（2） A model of duodenal ulcer induced by cysteine

　　1. Animal modeling materials: Female rats, weighing 200-250g; Medication: Cysteine.

　　2. Moulding method: Moulding rats should drink water containing cysteine at a daily dose of 50mg/100g for 1-3 months. Alternatively, inject 10 to 30mg/100g subcutaneously, once every 6 hours, and the mold can be formed after 4 days.

　　3. Modeling principle: Cysteine causes duodenal ulcers in animals.

　　4. Changes after modeling: During dissection, local adhesion with the liver, pancreas, or greater omentum can be observed, presenting as a penetrating ulcer lesion.

　　（3） Gastric ulcer model induced by hot cauterization

　　1. Model making material animals: rats, weighing 200-250g; Medication: Anesthetics; Equipment: Electric soldering iron.

　　2. Modeling method: After anesthesia, the rats were dissected under sterile conditions and heated to about 45 ℃ with a 3mm thick 15W electric soldering iron. After about 5 seconds of cauterization on the gastric wall or directly on the mucosa, transparent white spots were visible at the burn site. After a short wait, edema appeared in the mucosa, and after 6 hours, there was significant local bleeding.

　　3. Modeling principle: Burns can cause gastric ulcers in animals.

　　4. After a few days of modeling, the mucosa at the burn site developed ulcers, with granulation tissue and white fur on the wound surface. The gastric ulcer caused by this method usually recovers after 2-4 weeks. To prolong the course of the disease, cortisone can be administered orally after hot cauterization, with a daily dose of 75mg/kg. Continuous application can prolong the pathological process and even keep the gastric ulcer for 200 days.