动物造模,系统性红斑狼疮 z-bio 2023-09-13 999

　　1. The drug lupus model commonly uses a high concentration of isopentadiene type alkane - pristane - enriched in mineral oil, and intraperitoneal injection of pristane is a standard method for obtaining enriched monoclonal antibody ascites. The use of hypoglycan in BALB/c mice can cause lupus characteristic autoantibodies, such as anti RNP antibodies, anti DNA, and anti histone antibodies, with antibody levels comparable to those of MRL/lpr mouse models. After the application of hypoglycemic agents, it was also found that immune complexes were deposited in the kidneys of mice, which directly led to severe proteinuria and nephritis. In addition to BALB/c mice, all other types of mice are also sensitive to chloramphenicol to varying degrees. In terms of gender differences, female lupus models induced by chloramphenicol are more severe than male models, at least in the SJL/J strain of mice. A BALB/c mouse model of systemic lupus erythematosus can be induced by a single intraperitoneal injection of 0.5ml of chloramphenicol.

　　2. The lymphocyte immune model is more commonly used for chronic graft-versus-host disease (cGVHD) models. The cGVHD model can be established by infusing lymphocytes from parental DBA mice into the F1 generation of hybrid mice (C57BL6 and DBA). Its characteristic is that the CD4+mediated response of the graft leads to host B cell activation, polyclonal proliferation, and the production of immunoglobulins. Some of the manifestations of this chronic disease model are similar to those of SLE, including ANA, lupus specific autoantibodies, and immune complex mediated nephritis. Another characteristic of this model is that the female phenotype is heavier than the male phenotype, which can be used to study gender differences in lupus incidence.

　　Dong et al. in China successfully established a mouse lupus nephritis model using the above method. The method is to randomly divide F1 hybrid mice into two groups. Under aseptic conditions, remove the spleen, lymph nodes, and thymus of the parental DBA mice, cut them into pieces, grind the slides, and rinse them with PBS. After sieving, the cell suspension is slowly added to the mouse lymphocyte separation solution along the offline tube wall in a 1:1 ratio. The lymphocyte layer is aspirated at 1800 rpm for 20 minutes, and diluted and washed with PBS three times. The first time is 1800 rpm for 10 minutes, followed by 1800 rpm for 5 minutes. Finally, an appropriate and well mixed lymphocyte suspension is prepared using PBS. Cell viability was measured using trypan blue exclusion test and phase microscope observation, and at least 90% of living cells were observed. The total number of cells was measured by flow cytometry. On days 0, 3, 7, and 10, the model group was injected with lymphocyte suspension through the tail vein of the recipient mice, with 0.25ml of 50 cells injected each time × 1000 000 receptor living cells. The control group was injected with an equal amount of PBS each time.