动物造模,饮食偏嗜法脾气虚证 z-bio 2023-09-13 835

　　(1) Reproduction method: Adult rats were orally gavaged with Shanxi white vinegar at a dose of 10ml/kg body weight for 10 consecutive days. Observe changes in food intake, spontaneous activity, body weight, rectal temperature, stool characteristics, and general condition. On the 16th day, each rat was given a 10% D-xylose solution of 10ml/kg body weight by gavage. After 1 hour, blood was collected from the femoral vein to detect changes in serum D-xylose, serum amylase, total serum protein, albumin, red blood cells (RBC), hemoglobin (Hb), white blood cells (WBC), platelets (PLT), E-rosette rate, and T-lymphocyte conversion rate. Rats were anesthetized with ether, and live gastric and jejunal segments were taken for electron microscopy and transmission electron microscopy observation; After the neck was cut off and executed, an autopsy was performed, and the stomach, jejunum, thymus, and spleen were taken for observation under a light microscope.

　　(2) Model characteristics: Starting from the 3rd day, the animal exhibits erectile hair, loss of luster in the coat, arched back, decreased activity, and abdominal distension. Subsequently, the coat withers, curls up, drowsiness, significantly reduced activity, limb fatigue, significant abdominal distension, small and dry stools or loose stools, perianal dirt, reduced food intake, light body weight, and no significant changes in body temperature. The levels of D-xylose and amylase in serum were significantly reduced. On autopsy, congestion and edema of the stomach, duodenum, and jejunum can be observed, especially with obvious jejunitis, intestinal distension, and intragastric bleeding. Histopathology shows atrophy and detachment of the gastric mucosa, congestion and edema of the submucosa, infiltration of inflammatory cells, and gastric bleeding; Jejunal mucosal atrophy, inflammation, detachment, and submucosal edema; The microvilli on the surface of gastric mucosal epithelial cells shed and became shorter, with a decrease in enzyme granules; The microvilli at the top of the absorption epithelial cells in the jejunum swell, detach, and decrease in number, with disordered arrangement and reduced surface sugar coating. Mitochondria swell, break, and decrease in number. The E-rate and lymphocyte conversion rate of animals were significantly reduced; The thymic corpuscles, thymic PAS substances, and spleen T and B cell regions were significantly reduced; There were no significant changes in serum total protein, albumin, RBC, Hb, WBC, and PLT.

　　(3) According to the theory of comparative medicine in traditional Chinese medicine, the five flavors enter the five organs, preferring the five flavors and damaging them. Based on this, many methods have been developed to create animal models of spleen deficiency syndrome using partial diet, including partial diet vinegar method, partial diet wine vinegar method, and partial diet bitter taste method. The animal model of spleen qi deficiency syndrome with a preference for sour taste is based on the animal model of spleen qi deficiency syndrome with a preference for alcohol, vinegar, and Shanxi white vinegar as the inducing factor. The animal model of spleen qi deficiency syndrome with a preference for sour taste has symptoms such as reduced food intake, abnormal bowel movements, reduced activity frequency, and weight loss. The model is stable, reliable, and has a high repetition rate.