动物造模,高血压肝阳上亢证动物模型 z-bio 2023-09-13 869

　　(1) Reproduction method: Adult rats were anesthetized, and a longitudinal incision was made in the middle of the abdomen. The bilateral renal arteries were bluntly separated. Silver clips with an inner diameter of 0.2-0.23mm were used to clamp the initial part of the bilateral renal arteries, and 40000 to 80000 U of penicillin was administered to prevent infection. Then, the abdomen was closed layer by layer. After 2 weeks of surgery, the wound healed completely and Fu Zi Tang (containing raw medicine 0.1g/ml) was administered by gavage, 20ml/(kg body weight · d), for 4 consecutive weeks. Observe the changes in appearance, temperament, water intake, rotation time, blood pressure, and other factors of rats. After 8 weeks, blood was taken to measure plasma NE and E, the animals were decapitated and killed, and the brainstem was separated to measure NE and 5-HT.

　　(2) The characteristics of the model are that the animal's fur color gradually loses its luster, becomes thin, thirsty, and the amount of water consumed significantly increases. The rotation time is significantly shortened (indicating dizziness), and there is a change in temperament from the addition of aconite soup. It reaches its peak at the 6th week and maintains until the end of the 8th week. The color of the combined membrane in the animal's eyes deepens and turns red, resulting in convulsions or hemiplegia. From the first week after surgery, blood pressure rapidly increased, and continued to rise steadily until the third week. It reached its peak at the 6th week and continued until the end of the 8th week. Plasma NE, E, and brainstem NE increased, while brainstem 5-HT significantly decreased.

　　(3) Comparative medicine liver yang hyperactivity syndrome refers to the syndrome of liver and kidney yin deficiency, which is caused by yin deficiency and yang hyperactivity and is characterized by dizziness, swelling and pain in the head, weight on the head and feet, and soreness and weakness in the waist and knees. A Double Kidney and Double Clamp Renal Vascular Hypertension Model in Xu

　　Similar to human hypertension in many aspects, blood pressure rises quickly and steadily, with a large amplitude; It is prone to stroke and has a development process similar to that of traditional Chinese medicine from liver yang hyperactivity (hypertension) to liver yang conversion wind (stroke). Aconite is a product of pungent, sweet, and high heat. When used in model animals, aconite will inevitably consume essence and blood, seize the yin of the liver and kidney, and cause liver and kidney yin deficiency, exacerbating liver yang hyperactivity. During the modeling process, the animals gradually developed a darker and redder color of the conjunctival membrane in both eyes, thirst, increased water intake, resistance to intense, screaming, jumping, and frequent fighting, biting, and other aggressive behaviors of rats in the same cage when lifting the tail, grasping the neck, or gently clamping. The rotation time was significantly shortened (indicating dizziness), and a few animals also experienced hemorrhagic stroke, which is consistent with the development process from liver yang hyperactivity to liver yang wind. It can be seen that this model has certain clinical features similar to the human liver yang hyperactivity syndrome, but the surgical damage of this model is relatively large and easy to cause animal death, but it is relatively inexpensive.