动物造模,胃黏膜损伤模型 z-bio 2023-09-13 993

　　1. Animal modeling materials: Healthy SD male rats, weighing 180-220g; Medication: Indomethacin.

　　2. Modeling method: After fasting for 18 hours and water deprivation for 1 hour, rats were gavaged with 0.1% indomethacin 5ml/kg. The control group was given an equal amount of physiological saline.

　　3. Modeling principle: Non steroidal anti-inflammatory drugs mainly inhibit the synthesis of prostaglandins with protective effects on gastric mucosal cells, induce mucosal lesions or promote the occurrence of peptic ulcers.

　　4. Visual observation of changes in gastric mucosa after modeling: The control group's gastric mucosa had a ruddy color and no damage was observed. The gastric mucosa in the model group was significantly congested, with extensive patchy bleeding and erosion lesions, scattered ulcers, and clots visible in the gastric cavity. In some rats, blood accumulation was observed in the gastric and intestinal cavities.

　　Light microscopy observation: The model group showed widespread degeneration of mucosal epithelial cells, extensive dilation and congestion of capillaries, and patchy or focal bleeding in both the mucosal layer and submucosal layer, with neutrophil infiltration and edema changes. In severe cases, it affected or exceeded the mucosal muscle layer; The average injury index showed a significant difference compared to the control group.

　　Scanning electron microscopy observation: The gastric mucosa of the control group rats was smooth and slightly undulating under the scanning electron microscope, with epithelial cells arranged regularly and covered with a thin layer of continuous mucus; At high magnification, the surface of cells can be seen to be rough, with more particles (glycoproteins secreted by cells) and mucinous filaments. Under scanning electron microscopy, the model group showed thinning of the mucus layer, damage to the mucosal surface cells, detachment of glandular cells, and widespread detachment of epithelial cells in some areas, with disordered cell arrangement and obvious bleeding.

　　Changes in gastric mucosal blood flow: Compared with the normal control group and the model group, the latter showed a significant decrease.

　　6-Ketone Prostaglandin F1 in Gastric Mucosal Tissue α (6-keto-PGF1 α)， Thromboxane B2 (TXB2)

　　Content change: The model group showed a significant decrease compared to the control group.